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px459v2 0 espcas9 expression vector  (Addgene inc)


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    Structured Review

    Addgene inc px459v2 0 espcas9 expression vector
    Px459v2 0 Espcas9 Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/px459v2+0+espcas9+expression+vector/pX459V2%2E0-eSpCas9(1%2E1)+(Plasmid+%23108292)/pmc10769860__pnas__2307395120__sapp-94-11-14
    Average 93 stars, based on 15 article reviews
    px459v2 0 espcas9 expression vector - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Secreted stromal protein ISLR promotes intestinal regeneration by suppressing epithelial Hippo signaling
    Article Snippet: .. Gene‐specific sgRNA oligos were cloned into the px459v2.0‐eSpCas9 expression vector (Addgene), which bicistronically expressed sgRNA and Cas9 nuclease. ..

    Article Title: Secreted stromal protein ISLR promotes intestinal regeneration by suppressing epithelial Hippo signaling
    Article Snippet: .. Genome engineering Gene‐specific sgRNA oligos were cloned into the px459v2.0‐eSpCas9 expression vector (Addgene), which bicistronically expressed sgRNA and Cas9 nuclease. ..

    Expressing:

    Article Title: Secreted stromal protein ISLR promotes intestinal regeneration by suppressing epithelial Hippo signaling
    Article Snippet: .. Gene‐specific sgRNA oligos were cloned into the px459v2.0‐eSpCas9 expression vector (Addgene), which bicistronically expressed sgRNA and Cas9 nuclease. ..

    Article Title: Secreted stromal protein ISLR promotes intestinal regeneration by suppressing epithelial Hippo signaling
    Article Snippet: .. Genome engineering Gene‐specific sgRNA oligos were cloned into the px459v2.0‐eSpCas9 expression vector (Addgene), which bicistronically expressed sgRNA and Cas9 nuclease. ..

    Article Title: Supplementary Information for Excessive nucleic acid R-loops induce mitochondria-dependent epithelial cell necroptosis and drive spontaneous intestinal inflammation
    Article Snippet: Enzyme-linked Immunosorbent Assay Serum levels of CRP, IL-6 and TNF-α were measured from mouse blood using enzyme-linked immunosorbent assay (ELISA) kit (MLBIO, ml064282, ml098430 and mlC50536, respectively) according to manufacturer’s instructions. .. CRISPR‒Cas9 constructs were generated by cloning double-stranded sgRNA oligos into the px459v2.0-eSpCas9 expression vector (Addgene, Cambridge, MA, USA). ..

    Plasmid Preparation:

    Article Title: Secreted stromal protein ISLR promotes intestinal regeneration by suppressing epithelial Hippo signaling
    Article Snippet: .. Gene‐specific sgRNA oligos were cloned into the px459v2.0‐eSpCas9 expression vector (Addgene), which bicistronically expressed sgRNA and Cas9 nuclease. ..

    Article Title: Secreted stromal protein ISLR promotes intestinal regeneration by suppressing epithelial Hippo signaling
    Article Snippet: .. Genome engineering Gene‐specific sgRNA oligos were cloned into the px459v2.0‐eSpCas9 expression vector (Addgene), which bicistronically expressed sgRNA and Cas9 nuclease. ..

    Construct:

    Article Title: Supplementary Information for Excessive nucleic acid R-loops induce mitochondria-dependent epithelial cell necroptosis and drive spontaneous intestinal inflammation
    Article Snippet: Enzyme-linked Immunosorbent Assay Serum levels of CRP, IL-6 and TNF-α were measured from mouse blood using enzyme-linked immunosorbent assay (ELISA) kit (MLBIO, ml064282, ml098430 and mlC50536, respectively) according to manufacturer’s instructions. .. CRISPR‒Cas9 constructs were generated by cloning double-stranded sgRNA oligos into the px459v2.0-eSpCas9 expression vector (Addgene, Cambridge, MA, USA). ..

    Generated:

    Article Title: Supplementary Information for Excessive nucleic acid R-loops induce mitochondria-dependent epithelial cell necroptosis and drive spontaneous intestinal inflammation
    Article Snippet: Enzyme-linked Immunosorbent Assay Serum levels of CRP, IL-6 and TNF-α were measured from mouse blood using enzyme-linked immunosorbent assay (ELISA) kit (MLBIO, ml064282, ml098430 and mlC50536, respectively) according to manufacturer’s instructions. .. CRISPR‒Cas9 constructs were generated by cloning double-stranded sgRNA oligos into the px459v2.0-eSpCas9 expression vector (Addgene, Cambridge, MA, USA). ..

    Cloning:

    Article Title: Supplementary Information for Excessive nucleic acid R-loops induce mitochondria-dependent epithelial cell necroptosis and drive spontaneous intestinal inflammation
    Article Snippet: Enzyme-linked Immunosorbent Assay Serum levels of CRP, IL-6 and TNF-α were measured from mouse blood using enzyme-linked immunosorbent assay (ELISA) kit (MLBIO, ml064282, ml098430 and mlC50536, respectively) according to manufacturer’s instructions. .. CRISPR‒Cas9 constructs were generated by cloning double-stranded sgRNA oligos into the px459v2.0-eSpCas9 expression vector (Addgene, Cambridge, MA, USA). ..



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